The Effects of (Otostegia Persica) Hydroalcoholic Extract on VEGF Receptor Gene Expression Changes in the Angiogenesis in Chick Chorioalantoic Membrane

Introduction: Angiogenesis is the formation of new vessels from early vessels that has effected in pathologic condition such as growing tumor, metastases, arthritis rheumatoid, diabetes and physiologic condition such as developmental and growing of organ regeneration, wound healing, and reproduction. Otostegia presica is a member of Laminacea family, has antioxidant and anti-apoptosis properties. The aim of this study was to investigate the effects of hidroalkoholic extract of Otostegia presica on VEGFR expression and angiogenesis in chick chorioallantoic membrane. Methods: In this research, 60 fertilized Ross eggs were used and randomly were divided into five groups, included: control, sham-exposed, and three experimental groups treated with hidroalkoholic extract of Otostegia presica doses 100,200,400 mg/kg. On 2nd day of incubation, a window was opened for eggs under sterile condition. On 8th day, a gelatin sponge with 1×4×4 diameter was placed on chorioalantoic membrane (CAM) and extract fractions with dose of 100,200,400 mg/kg were added. On 12th day, length and weight of embryos were measured and CAM photos were captured. Then number and length of vessels in special area on CAM were measured with Image J. software. Sampling was performed for assessment of VEGFR expression. Data were analyzed by t-test and ANOVA and p<0.05 was considered significant. Results: Comparison between mean of length and weight of embryos and number and length of vessels in controls and sham-exposed did not show significant difference (P>0.05). Mean of the number of vessels showed significant increase in all experimental groups (P<0.05). VEGFR expression has increased in experimental groups rather than sham-exposed group. Conclusion: The results showed that hidroalkoholic extract of Otostegia presica has have angiogenic effects and increased VEGFR expression in all experimental groups